Detection & Quantification of Microorganisms in Dental Unit Waterlines
نویسندگان
چکیده
The aim of the study was to assess microbial quality of water in dental unit waterlines in a dental school at Mangalore. Methodology: Water samples were taken from tap water for control, air/water syringe and the high speed hand piece of 20 dental units. The samples were transferred to the laboratory and streaked on the culture media namely Mac Conkey agar, Sabourauds dextrose media, Buffered charcoal yeast extract agar base & Lactobacillus agar. The microorganisms were identified by gram staining followed by the biochemical methods and the number of colony forming units (CFU) was determined. Results: The results were tabulated using One-way ANOVA test. The maximum microorganisms found in the samples obtained from the handpiece belonged to the species in the following order Legionella (142.8 CFU), Lactobacillus spp (120.3 CFU) , Acinetobacter spp (93.25 CFU),Micrococcus spp (82.55 CFU), Staphylococcus spp (73.4 CFU), Pseudomonas aeruginosa (67.85 CFU), Klebsiella pneumonia (64.1 CFU), Streptococcus spp (56.8 CFU), Burkholderia cepacia(0 CFU). The samples obtained from the air/water syringe showed Legionella (116.45 CFU) ,Lactobacillus spp(115.2 CFU) , Acinetobacter spp(95.75 CFU) , Micrococcus spp (80.35 CFU),Staphylococcus spp (70.85 CFU),Klebsiella pneumonia(62.6 CFU), Pseudomonas aeruginosa (52.9 CFU) ,Streptococcus spp (52.35 CFU), Burkholderia cepacia (0 CFU).The samples obtained from the control group showed Legionella (160.55 CFU), Lactobacillus spp (140.9 CFU), Acinetobacter spp (130.95 CFU),Micrococcus spp (93.35 CFU),Staphylococcus spp (84.15 CFU), Pseudomonas aeruginosa (94.7 CFU), Klebsiella pneumonia (75.4 CFU),Streptococcus spp (59.65 CFU), Burholderia cepacia (0 CFU) Conclusion: The Dental unit water lines showed microbial count well within the permissible limits specified by the American Dental Association (ADA).
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